DELFIA has a similar assay principle and workflow to that of a traditional ELISA, but with the added benefits of a stable, time-resolved fluorescent signal improving assay dynamic range. DELFIA also offers a multiplexing capability.
Immunoassay Platforms to Fit Every Need
Be sure to choose the best possible option for success
The right technology choice for a successful assay
When it comes to choosing the right assay for your research, traditional technology almost always gives you the choice between going with ELISA (enzyme-linked immunosorbent assays) or Western Blot.
While these techniques are quite well known and widely used, others can be implemented which will provide you with additional benefits like ease of use, scalability, or the absence of washing, without compromising on sensitivity and specificity.
DELFIA®, LANCE®, HTRF®, or Alpha technologies are among the many options to explore!
ELISA | DELFIA | HTRF and LANCE Ultra | Alpha | ||
---|---|---|---|---|---|
PLATE FORMAT | 96-wells | 96 to 384-wells | Flexible: Anywhere from 96- to 1536-wells | ||
MINIATURIZATION | Limited | Limited | Yes | Yes | |
TIME TO RESULTS | 4-24 hr | 4-6 hr | 1-2 hr | 1-2 hr | |
– | – | Significant time saving reallocated back to science | |||
WASH STEPS | Yes | Yes | No | No | |
While timely, in some instances wash steps can be beneficial. Wash steps may become necessary to remove nonbound analytes with higher cross-reactivity profiles and to decrease background | Absence of wash steps increases throughput, results in ease of use, reduces time to results and lowers variability | ||||
DYNAMIC RANGE | 2 Log | 5 Log | 4 Log | 5 Log | |
– | Large dynamic ranges are beneficial to avoid making extremely large dilutions during sample preparation. Large sample dilutions are cumbersome and lead to analytical imprecision/variability | ||||
SENSITIVITY | 10 pg/mL | 10 pg/mL | 10 pg/mL | 1 pg/mL | |
Sufficient sensitivity to assay in serum, fluid, cell supernatant & lysates | Sufficient sensitivity to assay in cell supernatant & lysates | Sufficient sensitivity to assay in cell supernatant & lysates. May lend itself to diluting then testing serum samples, due to its superior sensitivity | |||
AVIDITY | No | No | Only with streptavidin | Yes | |
– | – | Avidity may be a sought after characteristic when detecting protein-protein interactions of low affinities | |||
SIGNAL STABILITY | Up to 1 hr | Days – Years | 24 hr to days | up to 24 hr | |
– | Allows for the plates to be left on the bench and read at an alternate later and more convenient time. Maximizes assay precision when incubation times vary | – | |||
MULTIPLEXING | No | Yes (up to 3 targets) | No | Yes (up to 3 targets) | |
READER REQUIREMENTS | Chromogenic and Chemiluminescence Compatible | TR-FRET Compatible | TR-FRET Compatible | Alpha Compatible | |
– | 100+ compatible reader | 50+ compatible reader | 10+ compatible reader | ||
CHEMISTRY | – | Fluorophore Chelate | Fluorophore Chelate (LANCE Ultra) and Fluorophore Cryptate (HTRF) | Fluorophore Embedded Beads | |
– | Extremely robust chemistry, light, temperature insensitive. Ion insensitive (HTRF only) | – | |||
ANALYTE SIZE | No limitation | No limitation | Subject to förster radius (9 nm) | Subject to oxygen singlet diffusion (200 nm) |
DELFIA technology
DELFIA has a similar assay principle and workflow to that of a traditional ELISA, but with the added benefits of a stable, time-resolved fluorescent signal improving assay dynamic range. DELFIA also offers a multiplexing capability.
How DELFIA compares to ELISA
Below is a workflow comparison between a DELFIA TRF assay and a traditional ELISA.
LANCE Ultra technology
LANCE Ultra assay principle
It’s everything you love about TRF detection with faster time to results and no wash steps.
How LANCE Ultra compares to ELISA
Below is a workflow comparison between a LANCE Ultra TR-FRET assay and a traditional ELISA.
HTRF technology
HTRF assay principle
Rapid, homogeneous, easy to use and to automate, allowing extreme miniaturization and easy assay development – over time, HTRF has become the gold reference for time-resolved FRET.
HTRF technology can be used in competitive and noncompetitive formats, performed as cellular or biochemical assays and thus can been applied to a variety of applications including GPCRs, kinases, epigenetics, and quantification of a range of biomarkers including cytokines.
How HTRF compares to ELISA
Below is a workflow comparison between an HTRF assay and a traditional ELISA.
Alpha technology
Alpha assay principle
How Alpha compares to ELISA
Below is a workflow comparison between an Alpha assay and a traditional ELISA.