Highly compatible plates for HTRF®
The right plate for a successful assay
- FIA white plates from many suppliers remain the most appropriate standard. The signal to noise ratios obtained using white microplates do not differ greatly from those of black plates. Absolute counts, however, are significantly higher in
white plates, thereby improving count statistics and subsequent assay reproducibility and sensitivity. NOTE: The use of white plates is mandatory for some assays and highly recommended when detection is performed using certain instruments,
in particular on monochromator-based plate readers (Please refer to the package inserts).
- Background levels may differ substantially among various plate types. Tissue-culture treated, or surface-treated plates may also be used.
- Glass bottom plates produce suboptimal results and should not be used.
All the benefits of HTRF with ELISA plate ease of use
protocol with suspension cells or with a transfer step for adherent cells. These plates can be read on all HTRF certified readers, thus making their implementation in
your experiments really easy
|Provider||Format||Part Numbers||Recommended volume||Type|
|Cisbio||96-w low volume||6PL96005 – 66PL96025 – 66PL96100||20-30 µL||White|
|PerkinElmer||96-w||6005680 – 6005688 – 6005689||80-350 µL||White, TC-treated, Sterile|
|PerkinElmer||384-w||6007680 – 6007688 – 6007689||24-90 µL||White, TC-treated, Sterile|
|PerkinElmer||384-w shallow well||6008280 – 6008289||10-20 µL||White|
|PerkinElmer||384-w||6007290 – 6007299||24-90 µL||White|
Whatever the plate type, assay volume should match well volume as closely as possible so that focalization of the reader excitation beam gives maximum energy delivery (Fig. 1) . For instance, when assay volumes are equal to or lower than 20 µl,
it is better to use 384 low-volume or 1536-well plates rather than regular 384-well plates.
Matching culture containers with signal detection formats
In case your experimental or culture conditions require you cultivate your cells in a specific flask or plate format prior to performing an assay, you can still benefit from an optimal reading by adapting the transfer step between culture
format and 96w or 384w reading plate.