Human alpha-fetoprotein immunoradiometric assay kit RIA
The ELSA2-AFP kit is an immunoradiometric assay for alpha-fetoprotein (AFP)
and important marker in obstetrics and oncology—in serum, plasma, or amniotic fluid.
Diagnosis and monitor hepatocellular carcinomas, all teratocarcinomas, and liver metastases
use as indicator of intense liver regeneration in viral hepatitis
Differentially diagnose between biliary atresia and neonatal hepatitis and confirm hereditary tyrosinosis
Diagnosis fetal neural tube defects (anencephaly, spina bifida) in maternal serum and amniotic fluid samples taken between 16-18 weeks of pregnancy High levels indicate fetal distress or multiple pregnancy, and low levels toxemia, slow fetal growth or placental tumor
- ACCURATE AND RELIABLE ASSAY
Two monoclonal antibodies are used for the sandwich. The first monoclonal antibody is adsorbed onto the ELSA solid phase. The second monoclonal antibody, radiolabeled with iodine-125, is used as the tracer.
Assay procedure: 30 min at RT / shaking + 30 min at RT / shaking.
Working range: 0 – 800 ng/mL (1 ng CIS = 1 IU 1st IS 72/225)
Sample: serum, plasma or amniotic fluid – 50 µL
Analytical sensitivity: 0.5 ng/mL
Population n = 220 healthy subjects; mean = 2.55 ng/mL - SD = 1.58 ng/mL; 96.4% of the values = 6 ng/mL