Human IL4 kit
No-wash kit to quantify released Human IL4
Human IL23 kit HTRF®
The HTRF human IL23 kit is designed for the quantification of human IL23 release in cell supernatant.
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No-wash
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Highly specific
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Under 2h bench time
Overview
IL23 is part of the response against infection as it upregulates the expression of MMP9 (matrix metalloprotease 9), increases angiogenesis, and reduces CD8 T cell infiltration into tumors. In oncology and immuno-oncology, IL23 has been studied for its effects on T cell infiltration and its promotion on TH17 cell development. IL23 is a heterodimeric cytokine composed of p19 and p40 subunits, the latter being shared with IL12. Cisbio's Human IL23 assay is highly specific for IL23 and does not cross react with IL12.
Assessment of serum samples often requires enhanced sensitivity. In some cases, AlphaLISA assays may have sufficient sensitivity to enable the detection of low levels of analytes in serum or plasma. Check out the Human IL-23 alpha AlphaLISA kit’s analytical performances for more information or learn more about AlphaLISA. When assaying, always follow the recommended protocol and avoid highly haemolyzed samples.
Benefits
- SPECIFIC FOR IL23
- SCALABLE FROM 96 TO 1536
Assay principle
Cell supernatant, sample, or standard is dispensed directly into the assay plate for the detection by HTRF® reagents (384-well low-volume white plate or Cisbio low-volume 96-well plate in 20 µl). The antibodies labeled with the HTRF donor and acceptor are pre-mixed and added in a single dispensing step, to further streamline the assay procedure. The assay can be run up to a 1536-well format by simply resizing each addition volume proportionally.
Assay data analysis
The 4 Parameter Logistic (4PL) curve is commonly recommended for fitting an ELISA standard curve. This regression enables the accurate measurement of an unknown sample across a wider range of concentrations than linear analysis, making it ideally suited to the analysis of biological systems like cytokine releases.
To fully understand how to deal with HTRF data processing and also 4PL 1/y² fitting, please visit this page.
Cisbio also worked with Myassays.com to help you in your data analysis. By clicking on this link, you’ll be able to access a free online software to run your IL23 analysis.
Technical specifications of human IL23 kit
| Sample size | 16 µL |
| Final assay volume | 20 µL |
| Kit components | Lyophilized standard, frozen detection antibodies, buffers &protocol. |
| LOD &LOQ (in Diluent) | 30 pg/mL &92 pg/mL |
| Range | 92 30,000 pg/mL |
| Time to result | 3h at 4°C |
| Species | Human only |
Intra and inter assay
Intra-assay (n=24)
| Sample | Mean [IL23] (pg/mL) | CV |
|---|---|---|
| 1 | 200 | 10% |
| 2 | 10,000 | 2% |
| 3 | 20,000 | 2% |
| Mean CV | 5% |
Each of the 3 samples was measured 24 times, and % CV was calculated for each sample.
Inter-assay (n=4)
| Sample | [IL23] (pg/mL) | Mean (delta R) | CV |
|---|---|---|---|
| 1 | 307 | 259 | 12% |
| 2 | 1920 | 1534 | 7% |
| 3 | 12,000 | 8601 | 3% |
| Mean CV | 7% |
Each of the samples was measured in 4 different experiments, and % CV was calculated for each sample
In collaboration with CNRS - Scientific Presentations
HTRF Cytokine cell-based assays
Monitor immune regulation the simple way - Flyers
HTRF assays for Oncology and Inflammation
Signaling in the Immune System - Brochures
HTRF measurement of cytokine release from fresh blood samples
In collaboration with Blood assay solution - Application Notes
Cytokine secretion profile on PBMCs
One sample to measure them all - Posters
Guidelines from PBMC isolation to cytokine assay optimisation
Perform and optimize cytokine assays on PBMC - Technical Notes
Guideline for PBMC freezing - Technical Notes
How to measure multiple cytokines from 1 sample
What to expect at the bench - Videos
HTRF makes cytokine detection easy
A fun video introducing you to cytokines assays with HTRF - Videos
All your HTRF assays in one document! - Catalog
A guide to Homogeneous Time Resolved Fluorescence
General principles of HTRF - Guides
Cytokine assays: a guide to success with HTRF
Setting up a successful cytokine assay - Guides
Portfolio of Life Sciences Cytokine Assays
This leaflet presents the analytical performances of each assay - Brochures
How HTRF compares to Western Blot and ELISA
Get the brochure about technology comparison. - Brochures
Protocol for Th17 differentiation from CD4 mouse T-lymphocyte
A step by step protocol for Th17 cells differentiation from naive CD4 mouse cells - Technical Notes
Plate Reader Requirement
Choosing the right microplate reader ensures you’ll get an optimal readout. Discover our high performance reader, or verify if your lab equipment is going to be compatible with this detection technology.
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