MAb Anti-phospho AKT-Eu cryptate HTRF®

MAb Anti-phospho AKT-Eu cryptate enables the rapid detection of Akt phosphorylation on Ser473 in a biochemical kinase assay format.
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  • No-wash No-wash
  • Ease-of-use Ease-of-use
  • High affinity High affinity
MAb Anti-phospho AKT-Eu cryptate enables the rapid detection of Akt phosphorylation on Ser473 in a biochemical kinase assay format.


The Anti-phospho AKT-Eu cryptate is a labeled monoclonal antibody enabling the rapid detection of Akt only when phosphorylated at Ser473. This allows the construction of a highly specific biochemical kinase assay. Any kind of substrate can be used, from small synthetic peptides to natural substrates, meaning high assay detection versatility. As HTRF tolerates extremely high ATP concentrations, an assay can be performed at physiological ATP concentrations.



Assay principle

HTRF kinase assays typically use a combination of a Europium-cryptate labeled anti-phosphoresidue antibody, SA-XL665 and a biotinylated* substrate. The signal is proportional to the concentration of phospho-residues. *Alternatively, other tags such as 6HIS, GST, c-myc, DNP, or FLAG may be used to label the kinase substrate.
HTRF kinase assay principle

Assay protocol

HTRF kinase assays have two basic phases: 1. Enzymatic step: biotinylated substrate is incubated with the kinase of interest and with the compounds in presence of co-factors. The reaction starts with the addition of ATP. 2. Detection step: The phosphorylated biotinylated substrate is then detected by the addition of Streptavidin-XL665 and MAb Anti-phospho AKT-Eu cryptate , prepared in a buffer containing EDTA to stop the enzymatic reaction.
HTRF kinase assay protocol

Open R&D: Sanofi Access Platform

In collaboration with Sanofi - Scientific Presentations

Experience the freedom of building your own assay

HTRF kinase activity toolbox reagents - Flyers

Product Insert A-P AKT-Eu / 61P15KAE-61P15KAZ-61P15KAY

61P15KAE-61P15KAZ-61P15KAY - Product Insert

HTRF Product Catalog

All your HTRF assays in one document! - Catalog

A guide to Homogeneous Time Resolved Fluorescence

General principles of HTRF - Guides

The essential white paper to expand your knowledge on kinases

Learn about cutting edge kinase research in this free white paper - Guides

How HTRF compares to Western Blot and ELISA

Get the brochure about technology comparison. - Brochures

Revolutionize your kinases investigations with HTRF

Watch this video to learn more about kinases investigations - Videos

Easy method for kinetic binding determination

How to revolutionize your kinetic binding demonstration with HTRF kinase binding platform assays - Application Notes

Safety Data Sheet A-P AKT-Eu / 61P15KAE

61P15KAE - Safety Data Sheet

Batch Information A-P AKT-Eu / 20230724

61P15KAE Batch 08A - Batch Information

Safety Data Sheet A-P AKT-Eu / 61P15KAY

61P15KAY - Safety Data Sheet

Safety Data Sheet A-P AKT-Eu / 61P15KAZ

61P15KAZ - Safety Data Sheet

Batch Information A-P AKT-Eu / 20230724

61P15KAZ Batch 08 - Batch Information

Plate Reader Requirement

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