MAP-Tau Kit
MAP-tau quantification in cell lysates
Phospho-alpha Synuclein (Ser129) cellular kit HTRF®
This HTRF kit enables the cell-based detection of phosphorylated alpha-synuclein at Ser129 as a marker of neurodegenerative diseases.
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All inclusive kit
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High sensitivity
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Faster and more convenient than ELISA
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No-wash
Overview
The HTRF Phospho-alpha Synuclein (Ser129) cell-based assay kit is ideal for quantifying endogenous phospho-alpha Synuclein phosphorylated on Serine 129.
The alpha Synuclein protein is involved in the pathogenesis of synucleinopathies, a group of neurodegenerative diseases that includes Parkinson’s disease (PD), dementia with Lewy bodies (DLB), diffuse Lewy body disease (DLBD), and multiple system atrophy (MSA).
Benefits
- SPECIFICITY
- PRECISION
Phospho-Synuclein (Ser129) assay principle
The Phospho-alpha Synuclein (Ser129) assay measures Synuclein when phosphorylated at Ser129. Unlike Western Blot, the assay is entirely plate-based and does not require gels, electrophoresis or transfer.
The Phospho-Synuclein (Ser129) assay uses 2 labeled antibodies: one with a donor fluorophore, the other with an acceptor. The first antibody was selected for its specific binding to the phosphorylated motif on the protein, the second for its ability to recognize the protein independent of its phosphorylation state. Protein phosphorylation enables an immune-complex formation involving both the labeled antibodies, and which brings the donor fluorophore into close proximity to the acceptor, thereby generating a FRET signal. Its intensity is directly proportional to the concentration of phosphorylated protein present in the sample, and provides a means of assessing the protein’s phosphorylation state under a no-wash assay format.
Phospho-alpha Synuclein (Ser129) 2-plate assay protocol
The 2 plate protocol involves culturing cells in a 96-well plate before lysis, then transferring lysates into a 384-well low volume detection plate before adding Phospho-alpha Synuclein (Ser129) HTRF detection reagents.
This protocol enables the cells' viability and confluence to be monitored.
Phospho-alpha Synuclein (Ser129) 1-plate assay protocol
Detection of Phospho-alpha Synuclein (Ser129) with HTRF reagents can be performed in a single plate used for culturing, stimulation, and lysis. No washing steps are required.
This HTS designed protocol enables miniaturization while maintaining robust HTRF quality.
Phosphorylation of alpha Synuclein on Serine 129 in Human samples
HEK293 cells were transfected with a plasmid encoding alpha-Synuclein prior to seeding treatment with non phosphorylated fibrils. After cell lysis, different dilutions of lysate were analyzed by HTRF.
Phosphorylation of alpha synuclein was detected in human brain homogenates from patients with Lewy Body Diseases.
Phosphorylation of alpha Synuclein on Serine 129 in Mouse samples
The alpha Synuclein phosphorylation was detected on primary neurons obtained from WT mouse .
Inducible recombinant mouse N2A cells treated with non phosphorylated fibrils displayed high phosphorylation levels of alpha Synuclein.
Parkinson's disease pathway and a-synuclein role
Alpha-Synuclein is a prominent component of intracellular fibrillary aggregates in the brains of patients suffering from synucleinopathies. This protein represents a key molecular hallmark for Parkinson’s disease, dementia with Lewy bodies, or multiple system atrophy. The extensive phosphorylation of alpha synuclein on Ser129 is also associated with a pathological event.
Changes in CREB and ERK phosphorylation levels after beta-amyloid treatment - Application Notes
Detection of human tau protein aggregation
For researcher working on PD or AD - Application Notes
Cell Signaling: Biomarkers, Phospho- & total-protein Assays - Flyers
Cell Signaling: Biomarkers, Phospho- & total-protein assays - Flyers
HTRF Alpha-tubulin Housekeeping kit
Properly interpret your compound effect - Application Notes
Optimize your HTRF cell signaling assays on tissues
HTRF and WB compatible guidelines - Technical Notes
HTRF cell-based phospho-protein data normalization
Valuable guidelines for efficiently analyzing and interpreting results - Application Notes
Unleash the potential of your phosphorylation research with HTRF
A fun video introducing you to phosphorylation assays with HTRF - Videos
How to run a cell based phospho HTRF assay
3' video to set up your Phospho assay - Videos
Key guidelines to successful cell signaling experiments
Mastering the art of cell signaling assays optimization - Guides
Exploring LRRK2 phosphorylation with HTRF® assays
LRRK2 kit, a game changer in neuroscience - Posters
Best practices for analyzing brain samples with HTRF® phospho assays for neurosciences
Insider Tips for successful sample treatment - Technical Notes
Exploring Synuclein phosphorylation and aggegation with HTRF assays
Alpha Synuclein kits, perfect tools to study synucleinopathy - Posters
All your HTRF assays in one document! - Catalog
A guide to Homogeneous Time Resolved Fluorescence
General principles of HTRF - Guides
Product Insert a-Syn P-S129 Kit / 6FSYNPEG-6FSYNPEH
6FSYNPEG-6FSYNPEH - Product Insert
How HTRF compares to Western Blot and ELISA
Get the brochure about technology comparison. - Brochures
Best practices for analyzing tumor xenografts with HTRF phospho assays
Protocol for tumor xenograft analysis with HTRF - Technical Notes
Multi-tissue cellular modeling and anlysis of insulin signaling - Posters
HTRF® cell signaling platform combined with iCell® Hepatocytes
A solution for phospho-protein analysis in metabolic disorders - Posters
HTRF phospho-assays reveal subtle drug-induced effects
Detailed protocol and direct comparison with WB - Posters
A single technology for 2D cells, 3D cells, and xenograft models - Posters
PI3K/AKT/mTor translational control pathway - Posters
Universal HTRF® phospho-protein platform: from 2D, 3D, primary cells to patient derived tumor cells
Analysis of a large panel of diverse biological samples and cellular models - Posters
From 2D, 3D cell cultures to xenografts: A smart HTRF platform to maximize anticancer drug discovery
One technology across all samples - Application Notes
HTRF phospho assays reveal subtle drug induced effects in tumor-xenografts
Tumor xenograft analysis: HTRF versus Western blot - Application Notes
HTRF phospho-total lysis buffer: a universal alternative to RIPA lysis buffers
Increased flexibility of phospho-assays - Application Notes
Simplified pathway dissection with HTRF phospho-assays and CyBi-felix liquid handling
Analyse of PI3K/AKT/mTor translational control pathway - Application Notes
Cell-based kinase assays in HTS ? potential and limitations for primary and secondary screening
In collaboration with Bayer - Scientific Presentations
How to run a cell based phospho HTRF assay
What to expect at the bench - Videos
Molecular basis of neuroinflammation and neurodegeneration diseases
The essential guide for extending your knowledge on the molecular mechanisms of neurodegenerative diseases - Guides
HTRF cellular phospho-protein assays
Physiologically relevant results fo fast flowing research - Flyers
Neurodegeneration and its main related diseases
Discover this infographic design on neurodegenerative diseases - Infographics
Assays for neurosciences research
Advance your research on neurodegenerative diseases - Flyers
Guidelines for Cell Culture and Lysis in Different Formats Prior to HTRF Detection
Seeding and lysing recommendations for a number of cell culture vessels. - Technical Notes
Assessment of drug efficacy and toxicity by combining innovative technologies
Combination of AlphaLISA®, HTRF®, or AlphaLISA® SureFire® Ultra™ immunoassays with the ATPlite™ 1step cell viability assay - Application Notes
Methodological Aspects of Homogeneous Time-Resolved Fluorescence (HTRF)
Learn how to reduce time and sample consumption - Application Notes
Safety Data Sheet (DEU) a-Syn P-S129 Kit / 6FSYNPEG
6FSYNPEG - Safety Data Sheet
Safety Data Sheet (ELL) a-Syn P-S129 Kit / 6FSYNPEG
6FSYNPEG - Safety Data Sheet
Safety Data Sheet (FRA-FR) a-Syn P-S129 Kit / 6FSYNPEG
6FSYNPEG - Safety Data Sheet
Safety Data Sheet (ITA) a-Syn P-S129 Kit / 6FSYNPEG
6FSYNPEG - Safety Data Sheet
Safety Data Sheet (SPA) a-Syn P-S129 Kit / 6FSYNPEG
6FSYNPEG - Safety Data Sheet
Safety Data Sheet (ENG-GB) a-Syn P-S129 Kit / 6FSYNPEG
6FSYNPEG - Safety Data Sheet
Safety Data Sheet (ENG-US) a-Syn P-S129 Kit / 6FSYNPEG
6FSYNPEG - Safety Data Sheet
Safety Data Sheet (DEU) a-Syn P-S129 Kit / 6FSYNPEH
6FSYNPEH - Safety Data Sheet
Safety Data Sheet (ELL) a-Syn P-S129 Kit / 6FSYNPEH
6FSYNPEH - Safety Data Sheet
Safety Data Sheet (FRA-FR) a-Syn P-S129 Kit / 6FSYNPEH
6FSYNPEH - Safety Data Sheet
Safety Data Sheet (ITA) a-Syn P-S129 Kit / 6FSYNPEH
6FSYNPEH - Safety Data Sheet
Safety Data Sheet (SPA) a-Syn P-S129 Kit / 6FSYNPEH
6FSYNPEH - Safety Data Sheet
Safety Data Sheet (ENG-GB) a-Syn P-S129 Kit / 6FSYNPEH
6FSYNPEH - Safety Data Sheet
Safety Data Sheet (ENG-US) a-Syn P-S129 Kit / 6FSYNPEH
6FSYNPEH - Safety Data Sheet
Safety Data Sheet (DEU) a-Syn P-S129 Kit / 6FSYNPEY
6FSYNPEY - Safety Data Sheet
Safety Data Sheet (ELL) a-Syn P-S129 Kit / 6FSYNPEY
6FSYNPEY - Safety Data Sheet
Safety Data Sheet (FRA-FR) a-Syn P-S129 Kit / 6FSYNPEY
6FSYNPEY - Safety Data Sheet
Safety Data Sheet (ITA) a-Syn P-S129 Kit / 6FSYNPEY
6FSYNPEY - Safety Data Sheet
Safety Data Sheet (SPA) a-Syn P-S129 Kit / 6FSYNPEY
6FSYNPEY - Safety Data Sheet
Safety Data Sheet (ENG-GB) a-Syn P-S129 Kit / 6FSYNPEY
6FSYNPEY - Safety Data Sheet
Safety Data Sheet (ENG-US) a-Syn P-S129 Kit / 6FSYNPEY
6FSYNPEY - Safety Data Sheet
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