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Phospho and Total protein blocking reagent HTRF®

This reagent is a component of total and phospho- protein kits and prevents protein degradation after cell lysis.
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This reagent is a component of total and phospho- protein kits and prevents protein degradation after cell lysis.
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In stock

Overview

Proteins and phosphoprotein may be prone to degradation following cell lysis, e.g. by endogeneous phosphatases. When running a cell-based assay, an appropriate stabilizing and blocking reagent is thus mandatory in order to prevent any remaining enzymatic activity, as well as to garantee protein integrity and quantification.

Benefits

  • SPECIFICITY
  • PRECISION

HTRF Product Catalog

All your HTRF assays in one document! - Catalog

A guide to Homogeneous Time Resolved Fluorescence

General principles of HTRF - Guides

How HTRF compares to Western Blot and ELISA

Get the brochure about technology comparison. - Brochures

Best practices for analyzing brain samples with HTRF® phospho assays for neurosciences

Insider Tips for successful sample treatment - Technical Notes

Optimize your HTRF cell signaling assays on tissues

HTRF and WB compatible guidelines - Technical Notes

Best practices for analyzing tumor xenografts with HTRF phospho assays

Protocol for tumor xenograft analysis with HTRF - Technical Notes

Key guidelines to successful cell signaling experiments

Mastering the art of cell signaling assays optimization - Guides

HTRF® cell signaling platform combined with iCell® Hepatocytes

A solution for phospho-protein analysis in metabolic disorders - Posters

HTRF phospho-assays reveal subtle drug-induced effects

Detailed protocol and direct comparison with WB - Posters

Universal HTRF® phospho-protein platform: from 2D, 3D, primary cells to patient derived tumor cells

Analysis of a large panel of diverse biological samples and cellular models - Posters

HTRF phospho assays reveal subtle drug induced effects in tumor-xenografts

Tumor xenograft analysis: HTRF versus Western blot - Application Notes

HTRF cell-based phospho-protein data normalization

Valuable guidelines for efficiently analyzing and interpreting results - Application Notes

HTRF phospho-total lysis buffer: a universal alternative to RIPA lysis buffers

Increased flexibility of phospho-assays - Application Notes

HTRF Alpha-tubulin Housekeeping kit

Properly interpret your compound effect - Application Notes

Simplified pathway dissection with HTRF phospho-assays and CyBi-felix liquid handling

Analyse of PI3K/AKT/mTor translational control pathway - Application Notes

Unleash the potential of your phosphorylation research with HTRF

A fun video introducing you to phosphorylation assays with HTRF - Videos

How to run a cell based phospho HTRF assay

3' video to set up your Phospho assay - Videos

How to run a cell based phospho HTRF assay

What to expect at the bench - Videos

HTRF cellular phospho-protein assays

Physiologically relevant results fo fast flowing research - Flyers

Guidelines for Cell Culture and Lysis in Different Formats Prior to HTRF Detection

Seeding and lysing recommendations for a number of cell culture vessels. - Technical Notes

Assessment of drug efficacy and toxicity by combining innovative technologies

Combination of AlphaLISA®, HTRF®, or AlphaLISA® SureFire® Ultra™ immunoassays with the ATPlite™ 1step cell viability assay - Application Notes

Methodological Aspects of Homogeneous Time-Resolved Fluorescence (HTRF)

Learn how to reduce time and sample consumption - Application Notes

Batch Information P+T protein BlockRgt / 20220919

64KB1AAC Batch 23A - Batch Information

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Batch Information P+T protein BlockRgt / 20230703

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Batch Information P+T protein BlockRgt / 20230703

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Batch Information P+T protein BlockRgt / 20230703

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Batch Information P+T protein BlockRgt / 20230919

64KB1AAC Batch 23C - Batch Information

Batch Information P+T protein BlockRgt / 20230703

64KB1AAD Batch 22A - Batch Information

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64KB1AAD Batch 25A - Batch Information

Batch Information P+T protein BlockRgt / 20250224

64KB1AAD Batch 26A - Batch Information

Plate Reader Requirement

Choosing the right microplate reader ensures you’ll get an optimal readout. Discover our high performance reader, or verify if your lab equipment is going to be compatible with this detection technology.

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