HTRF KinEASE STK Discovery kit
A assay to determine which HTRF KinEASE STK kit must be used
PolyGT-Biotin substrate HTRF®
PolyGT-Biotin has been shown to be a substrate for tyrosine-specific protein kinases.
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No-wash
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Ease-of-use
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Highly specific
Overview
Poly-(Glu,Tyr) (4:1) is a random copolymer which has been shown to be a substrate for tyrosine-specific protein kinases. Poly-(GT)-biotin may be used with Streptavidin-XL665 and Anti-phosphotyrosine-cryptate for easily optimized HTRF® tyrosine kinase assays. HTRF enables to perform assay at physiological ATP concentrations.
Benefits
- NO LIMITATION IN ATP CONCENTRATIONS
- RAPID ASSAY SETUP
- PHOSHO-(TYR) SPECIFIC
Assay principles
HTRF Tyrosine kinase assay use typically a combination of a Europium-cryptate labeled anti-phosphoresidue antibody, SA-XL665 and PolyGT-Biotin as substrate. The signal is proportional to the concentration of phospho-tyrosine.
Assay protocol
HTRF tyrosine kinase assay have two basic phases: 1. Enzymatic step: biotinylated PolyGT is incubated with the kinase of interest and with the compounds in presence of co-factors. The reaction starts with the addition of ATP. 2. Detection step: The phosphorylated biotinylated PolyGT is then detected by the addition of Streptavidin-XL665 and a Europium-cryptate labeled anti-phosphotyrosine antibody (MAb PY20-Eu cryptate or MAb PT66-Eu cryptate) prepared in a buffer containing EDTA to stop the enzymatic reaction.
Open R&D: Sanofi Access Platform
In collaboration with Sanofi - Scientific Presentations
Experience the freedom of building your own assay
HTRF kinase activity toolbox reagents - Flyers
Product Insert Poly GT / 61GT0BLA-61GT0BLD
61GT0BLA-61GT0BLD - Product Insert
All your HTRF assays in one document! - Catalog
A guide to Homogeneous Time Resolved Fluorescence
General principles of HTRF - Guides
The essential white paper to expand your knowledge on kinases
Learn about cutting edge kinase research in this free white paper - Guides
How HTRF compares to Western Blot and ELISA
Get the brochure about technology comparison. - Brochures
Revolutionize your kinases investigations with HTRF
Watch this video to learn more about kinases investigations - Videos
Easy method for kinetic binding determination
How to revolutionize your kinetic binding demonstration with HTRF kinase binding platform assays - Application Notes
Safety Data Sheet Poly GT / 61GT0BLA
61GT0BLA - Safety Data Sheet
Safety Data Sheet (DEU) Poly GT / 61GT0BLA
61GT0BLA - Safety Data Sheet
Safety Data Sheet (ELL) Poly GT / 61GT0BLA
61GT0BLA - Safety Data Sheet
Safety Data Sheet (FRA-FR) Poly GT / 61GT0BLA
61GT0BLA - Safety Data Sheet
Safety Data Sheet (ITA) Poly GT / 61GT0BLA
61GT0BLA - Safety Data Sheet
Safety Data Sheet (SPA) Poly GT / 61GT0BLA
61GT0BLA - Safety Data Sheet
Safety Data Sheet (ENG-GB) Poly GT / 61GT0BLA
61GT0BLA - Safety Data Sheet
Safety Data Sheet (ENG-US) Poly GT / 61GT0BLA
61GT0BLA - Safety Data Sheet
Batch Information Poly GT / 20230318
61GT0BLA Batch 32A - Batch Information
Safety Data Sheet Poly GT / 61GT0BLD
61GT0BLD - Safety Data Sheet
Safety Data Sheet (DEU) Poly GT / 61GT0BLD
61GT0BLD - Safety Data Sheet
Safety Data Sheet (ELL) Poly GT / 61GT0BLD
61GT0BLD - Safety Data Sheet
Safety Data Sheet (FRA-FR) Poly GT / 61GT0BLD
61GT0BLD - Safety Data Sheet
Safety Data Sheet (ITA) Poly GT / 61GT0BLD
61GT0BLD - Safety Data Sheet
Safety Data Sheet (SPA) Poly GT / 61GT0BLD
61GT0BLD - Safety Data Sheet
Safety Data Sheet (ENG-GB) Poly GT / 61GT0BLD
61GT0BLD - Safety Data Sheet
Safety Data Sheet (ENG-US) Poly GT / 61GT0BLD
61GT0BLD - Safety Data Sheet
Batch Information Poly GT / 20230318
61GT0BLD Batch 32A - Batch Information
Batch Information Poly GT / 20240622
61GT0BLD Batch 33A - Batch Information
Plate Reader Requirement
Choosing the right microplate reader ensures you’ll get an optimal readout. Discover our high performance reader, or verify if your lab equipment is going to be compatible with this detection technology.
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