A2BR expressing cells labeled with Terbium
Tag-lite cells transiently expressing the A2B receptor with Terbium
All information on this page pertains to the Tag-lite plasmid cloned with the Adenosine A2B receptor.
SNAP-tag® is a small fusion tag that covalently interacts with specific substrates. It enables the specific and covalent labeling of any protein of interest (refer to labeling procedure). Cells are provided unlabeled, and need to be labeled with Lumi4-Terbium prior to running a binding assay. Labeling reagents are available in 4 different sizes from the Cisbio catalog.
A saturation binding assay measures total and non-specific binding for increasing concentrations of ligand under equilibrium conditions. To perform the assay, the fluorescent ligand is titrated into a solution containing a fixed amount of labeled cells, and then incubated to equilibrium. The HTRF ratio obtained from this titration is the total binding.
A competitive binding assay is performed to measure the dissociation constant, Ki. To perform the assay, the compound is titrated into a solution containing a fixed concentration of fluorescent ligand and a fixed amount of cells.
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How to revolutionize your kinetic binding demonstration with HTRF kinase binding platform assays - Application Notes
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