Alpha-Tubulin Housekeeping Cellular Kit
Total NFkB cellular kit HTRF®
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Ready-to-use
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High sensitivity
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Faster and more convenient than ELISA
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No-wash
Overview
Total NFkB kit serves as a normalization assay with our Phospho-NFkB kits. NFkB (Nuclear Factor Kappa B) is of great interest in cancer research and a key player in the inflammatory response. The buffers of both HTRF phospho- and total NFkB assays are compatible, enabling an analysis of the phosphorylated and the total protein populations from one lysate sample.
Benefits
- SPECIFICITY
- PRECISION
- DATA NORMALIZATION
Total-NFkB assay principle
Total-NFkB 2-plate assay protocol
Total-NFkB 1-plate assay protocol
Detection of total NFkB in various human/mouse cells
HTRF assay vs WB using total-NFkB assay

TNFa dose-response on HeLa cells using phospho and total NFkB kit
Pharmacological response on Phospho and total NFkB of BAY 11-7085
Regulation of the NFkB pathway
Simplified pathway dissection with HTRF phospho-assays and CyBi-felix liquid handling
Analyse of PI3K/AKT/mTor translational control pathway - Application Notes
Open R&D: Sanofi Access Platform
In collaboration with Sanofi - Scientific Presentations
Cell Signaling: Biomarkers, Phospho- & total-protein Assays - Flyers
HTRF cellular phospho-protein assays
Physiologically relevant results fo fast flowing research - Flyers
Biomarker and Cell Signaling Assays for Fibrosis and NASH
HTRF solutions for NASH - Flyers
Cell Signaling: Biomarkers, Phospho- & total-protein assays - Flyers
HTRF assays for Oncology and Inflammation
Signaling in the Immune System - Brochures
PI3K/AKT/mTor translational control pathway - Posters
Universal HTRF® phospho-protein platform: from 2D, 3D, primary cells to patient derived tumor cells
Analysis of a large panel of diverse biological samples and cellular models - Posters
From 2D, 3D cell cultures to xenografts: A smart HTRF platform to maximize anticancer drug discovery
One technology across all samples - Application Notes
HTRF phospho assays reveal subtle drug induced effects in tumor-xenografts
Tumor xenograft analysis: HTRF versus Western blot - Application Notes
HTRF cell-based phospho-protein data normalization
Valuable guidelines for efficiently analyzing and interpreting results - Application Notes
HTRF phospho-total lysis buffer: a universal alternative to RIPA lysis buffers
Increased flexibility of phospho-assays - Application Notes
Multi-tissue cellular modeling and anlysis of insulin signaling - Posters
Best practices for analyzing brain samples with HTRF® phospho assays for neurosciences
Insider Tips for successful sample treatment - Technical Notes
HTRF Alpha-tubulin Housekeeping kit
Properly interpret your compound effect - Application Notes
Optimize your HTRF cell signaling assays on tissues
HTRF and WB compatible guidelines - Technical Notes
Key guidelines to successful cell signaling experiments
Mastering the art of cell signaling assays optimization - Guides
HTRF phospho-assays reveal subtle drug-induced effects
Detailed protocol and direct comparison with WB - Posters
A single technology for 2D cells, 3D cells, and xenograft models - Posters
Best practices for analyzing tumor xenografts with HTRF phospho assays
Protocol for tumor xenograft analysis with HTRF - Technical Notes
How to run a cell based phospho HTRF assay
What to expect at the bench - Videos
Unleash the potential of your phosphorylation research with HTRF
Unmatched ease of use, sensitivity and specificity assays - Videos
Cell-based kinase assays in HTS ? potential and limitations for primary and secondary screening
In collaboration with Bayer - Scientific Presentations
STING HTRF offer to bridge innate and adaptive immunity
cGAS-STING signaling pathway from A to Z - Brochures
Product Insert NFkB total Kit / 64NFTPEG-64NFTPEH
64NFTPEG-64NFTPEH - Product Insert
All your HTRF assays in one document! - Catalog
A guide to Homogeneous Time Resolved Fluorescence
General principles of HTRF - Guides
Novel HTRF platform to delineate STING pathway
Explore the whole STING pathway with a single technology - Posters
How HTRF compares to Western Blot and ELISA
Get the brochure about technology comparison. - Brochures
STING, the next candidate for cancer immunotherapy
Infographic about STING protein - Infographics
A Guide To Current Therapies in Immuno Oncology
Clear overview of past, present and future of immunotherapy - Guides
HTRF® cell signaling platform combined with iCell® Hepatocytes
A solution for phospho-protein analysis in metabolic disorders - Posters
Unleash the potential of your phosphorylation research with HTRF
A fun video introducing you to phosphorylation assays with HTRF - Videos
How to run a cell based phospho HTRF assay
3' video to set up your Phospho assay - Videos
Characterize STING agonists with HTRF human IFNb
Data about HTRF IFNb correlation with gene reporter assay and ELISA - Application Notes
HTRF HMGB1, IL-1β and IL-18 assays discriminate between inflammasome triggered pyroptosis, and necroptosis - Application Notes
Neuroinflammation study combining hiPSC-derived astrocytes and HTRF
See published experiments and data demonstrating how immunoassays rise to the challenge of astrocyte studies in neuroinflammation research - Application Notes
On-demand webinar: Linking Neuroinflammation and Neurodegeneration
New insight into neuroinflammation research - Videos
Assays for autoimmunity research
Advance your research on autoimmune diseases - Flyers
Assays for neurosciences research
Advance your research on neurodegenerative diseases - Flyers
Advance your research on Fibrosis
Kits and reagents for Fibrosis research - Flyers
Guidelines for Cell Culture and Lysis in Different Formats Prior to HTRF Detection
Seeding and lysing recommendations for a number of cell culture vessels. - Technical Notes
Assessment of drug efficacy and toxicity by combining innovative technologies
Combination of AlphaLISA®, HTRF®, or AlphaLISA® SureFire® Ultra™ immunoassays with the ATPlite™ 1step cell viability assay - Application Notes
Methodological Aspects of Homogeneous Time-Resolved Fluorescence (HTRF)
Learn how to reduce time and sample consumption - Application Notes
Safety Data Sheet NFkB total Kit / 64NFTPEG
64NFTPEG - Safety Data Sheet
Safety Data Sheet (DEU) NFkB total Kit / 64NFTPEG
64NFTPEG - Safety Data Sheet
Safety Data Sheet (ELL) NFkB total Kit / 64NFTPEG
64NFTPEG - Safety Data Sheet
Safety Data Sheet (FRA-FR) NFkB total Kit / 64NFTPEG
64NFTPEG - Safety Data Sheet
Safety Data Sheet (ITA) NFkB total Kit / 64NFTPEG
64NFTPEG - Safety Data Sheet
Safety Data Sheet (SPA) NFkB total Kit / 64NFTPEG
64NFTPEG - Safety Data Sheet
Safety Data Sheet (ENG-GB) NFkB total Kit / 64NFTPEG
64NFTPEG - Safety Data Sheet
Safety Data Sheet (ENG-US) NFkB total Kit / 64NFTPEG
64NFTPEG - Safety Data Sheet
Safety Data Sheet NFkB total Kit / 64NFTPEH
64NFTPEH - Safety Data Sheet
Safety Data Sheet (DEU) NFkB total Kit / 64NFTPEH
64NFTPEH - Safety Data Sheet
Safety Data Sheet (ELL) NFkB total Kit / 64NFTPEH
64NFTPEH - Safety Data Sheet
Safety Data Sheet (FRA-FR) NFkB total Kit / 64NFTPEH
64NFTPEH - Safety Data Sheet
Safety Data Sheet (ITA) NFkB total Kit / 64NFTPEH
64NFTPEH - Safety Data Sheet
Safety Data Sheet (SPA) NFkB total Kit / 64NFTPEH
64NFTPEH - Safety Data Sheet
Safety Data Sheet (ENG-GB) NFkB total Kit / 64NFTPEH
64NFTPEH - Safety Data Sheet
Safety Data Sheet (ENG-US) NFkB total Kit / 64NFTPEH
64NFTPEH - Safety Data Sheet
Plate Reader Requirement
Choosing the right microplate reader ensures you’ll get an optimal readout. Discover our high performance reader, or verify if your lab equipment is going to be compatible with this detection technology.
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