Vasopressin V2R stably expressing cells
Tag-lite Vasopressin V2 stably expressing cells
Vasopressin V2R expressing cells labeled with Terbium HTRF®
Tag-lite cells transiently expressing the Vasopressin V2 receptor labeled with Terbium for use in receptor binding applications. The cells are provided ready to use.
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Fully functional cell line
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Transient expression
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Ready for binding
Overview
There are three subtypes of vasopressin receptor, V1A, V1B, and V2. The V2 receptor is mainly situated in the kidney and is important in free water reabsorption.
Cells expressing the V2 receptor are provided pre-labeled with Terbium, and can be used to conduct receptor binding studies on the aforementioned receptor.
Benefits
- PRE-LABELED WITH TERBIUM
- FROZEN CELLS, READY TO BE USED
- CELLS/LIGAND VALIDATED FOR BINDING
Assay principle
Running your V2 receptor binding assay using Tag-lite is as easy as it can gets. Simply dispense 10 µL of labeled cells into each well, followed by 5 µL of labeled ligand and 5 µL of the compound you wish to test. Like all HTRF assays, Tag-lite assays do not require any washing steps. A diagram of the procedure to be followed is given on the right.
Saturation binding (KD)
A saturation binding assay measures total and non-specific binding for increasing concentrations of ligand under equilibrium conditions. To perform the assay, the fluorescent ligand is titrated into a solution containing a fixed amount of labeled cells and then incubated to equilibrium. The HTRF ratio obtained from this titration is the total binding.
Watch this video explaining how to run a saturation binding assay using Tag-lite.
Competitive binding (KI)
A competitive binding assay is performed to measure the dissociation constant, Ki. To perform the assay, the compound is titrated into a solution containing a fixed concentration of fluorescent ligand and a fixed amount of cells.
Watch this video explaining how to run a competitive binding assay using Tag-lite.
Kd and Ki validation
Examples of data obtained using Vasopressin V2 labeled cells and their matching fluorescent ligand (L0063RED). Vasopressin was used as reference ligand. Results may vary from one HTRF® compatible reader to another.
Evaluation of a Tag-lite binding assay for a class B receptor
In collaboration with Boehringer Ingelheim - Scientific Presentations
Ultra HTS at Bayer: use of IP-One and Tag-lite assays in GPCR drug discovery
In collaboration with Bayer - Scientific Presentations
In collaboration with CNRS - Scientific Presentations
The easy way to approach biology without radioactivity - Flyers
Fluorescent Antagonists For Vasopressin V2GPCR: Ligand Screening and Oligomerization Assays
In collaboration with Université de Strasbourg, IGF CNRS INSERM - Posters
Challenge the limits of binding kinetics studies - Application Notes
Understanding GPCRs is the key to improved DD
In collaboration with GEN - Guides
Tag-lite, the easiest solution for studying GPCR-ligands interactions
The gold standard technology for receptor binding studies - Videos
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A look at the kinetics of cAMP production in cells - Application Notes
All your HTRF assays in one document! - Catalog
A guide to Homogeneous Time Resolved Fluorescence
General principles of HTRF - Guides
How HTRF compares to Western Blot and ELISA
Get the brochure about technology comparison. - Brochures
Webinar | Association Rate Constant: The Unsung Hero of Drug Discovery Kinetics
Available On-demand - Videos
Product Insert V2-R LabCell / C1TT1V2
C1TT1V2 - Product Insert
Easy method for kinetic binding determination
How to revolutionize your kinetic binding demonstration with HTRF kinase binding platform assays - Application Notes
A comprehensive overview of pharmacology's main ligands - Guides
GPCRs: the pathway to Discovery
Complete solutions for GPCR Drug Discovery - Brochures
Safety Data Sheet V2-R LabCell / C1TT1V2
C1TT1V2 - Safety Data Sheet
Safety Data Sheet (DEU) V2-R LabCell / C1TT1V2
C1TT1V2 - Safety Data Sheet
Safety Data Sheet (ELL) V2-R LabCell / C1TT1V2
C1TT1V2 - Safety Data Sheet
Safety Data Sheet (FRA-FR) V2-R LabCell / C1TT1V2
C1TT1V2 - Safety Data Sheet
Safety Data Sheet (ITA) V2-R LabCell / C1TT1V2
C1TT1V2 - Safety Data Sheet
Safety Data Sheet (SPA) V2-R LabCell / C1TT1V2
C1TT1V2 - Safety Data Sheet
Safety Data Sheet (ENG-GB) V2-R LabCell / C1TT1V2
C1TT1V2 - Safety Data Sheet
Safety Data Sheet (ENG-US) V2-R LabCell / C1TT1V2
C1TT1V2 - Safety Data Sheet
Plate Reader Requirement
Choosing the right microplate reader ensures you’ll get an optimal readout. Discover our high performance reader, or verify if your lab equipment is going to be compatible with this detection technology.
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